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The collection

by Frédérique Foudrinier last modified 03-04-2017 15:18

Description

The collection is conserved in double, a cryobank is located in Limoges, the other is in Reims.The strains of Toxoplasma gondii are obtained by mouse inoculation, or cell culture (THP1 or VERO), from samples of either human (cases of congenital toxoplasmosis, immunocompromised hosts or acquired toxoplasmosis), or animal origin. 

The collection is constituted by the stocks of strains (tachyzoites or cysts) preserved in liquid nitrogen. 

 
Typing of Toxoplasma gondii strains
 
The genetic characterization is performed with a high resolution method, based on the length polymorphism of 15 microsatellites markers (1).  
 
Furthermore, 19 strains available in the catalog of BRC Toxoplasma were the object of complete sequencing (2). The haplogroup membership was defined by sequencing of introns (3).
 
Souches catalogue séquencées
 
Click on this link to enlarge the image: sequencing
 
 

Bibliographic references :

  1. Ajzenberg D, Collinet F, Mercier A, Vignoles P, Dardé ML. Genotyping of Toxoplasma gondii isolates with 15 microsatellite markers in a single multiplex PCR assay. J Clin Microbiol. 2010 Dec ; 48 (12) : 4641-5. Epub 2010 Sep 29. PubMed : 20881166 ; PubMed Central : PMC3008440   

  2. Lorenzi H, Khan A, Behnke MS, Namasivayam S, Swapna LS, Hadjithomas M, Karamycheva S, Pinney D, Brunk BP, Ajioka JW, Ajzenberg D, Boothroyd JC, Boyle JP, Dardé ML, Diaz-Miranda MA, Dubey JP, Fritz HM, Gennari SM, Gregory BD, Kim K, Saeij JP, Su C, White MW, Zhu XQ, Rosenthal BM, Grigg ME, Parkinson J, Liu L, Kissinger JC, Roos DS, David Sibely L. Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes. Nat Commun. 2016 Jan 7; 7:10147. doi:10.1038/ncomms10147

  3. Su C, Khan A, Zhou P, Majumdar D, Ajzenberg D, Dardé ML, Zhu XQ, Ajioka JW, Rosenthal BM, Dubey JP, Sibley LD. Globally diverse Toxoplasma gondii isolates comprise six major clades originating from a small number of distinct ancestral lineages. Proc natl Acad Sci U S A. 2012 Apr 10; 109(15):5844-9. doi: 10.1073/pnas.1203190109



Conservation       
    
The storage of the strains in liquid nitrogen assure an optimum conservation.
To preserve the collection, frozen aliquots of tachyzoites and/or bradyzoites are stored in a double location in similar containers at Reims and at Limoges. 
 
Quality controls
 
All cell cultures are checked for Mycoplasma.
Before storage, genetic identification is verified for each stock of strain by microsatellite analysis.

 

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